Blebbing, free Ca2+ and mitochondrial membrane potential preceding cell death in hepatocytes
作者： John J. Lemasters , James DiGuiseppi , Anna-Liisa Nieminen , Brian Herman
DOI: 10.1038/325078A0
关键词: Viability assay 、 Bleb (cell biology) 、 Membrane potential 、 Intracellular 、 Cell biology 、 Video microscopy 、 Mitochondrion 、 Biology 、 Cytosol 、 Biochemistry 、 Programmed cell death
摘要: Cell surface 'blebbing' is an early consequence of hypoxic and toxic injury to cells. A rise in cytosolic free Ca2+ has been suggested as the stimulus for bleb formation final common pathway irreversible cell injury. Here, using digitized low-light video microscopy, we examine blebbing, Ca2+, mitochondrial membrane potential loss viability individual cultured hepatocytes. Unexpectedly, found that after 'chemical hypoxia' with cyanide iodoacetate, does not change during or before cellular viability. death was precipitated by a sudden breakdown plasma permeability barrier, possibly caused rupture bleb.
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