Peroxiredoxin 6 from cuttlefish ( Sepiella maindroni ): molecular characterisation during development and the response to thermal stress
作者： Weiwei Song , Chunlin Wang , Ronghua Li , Changkao Mu
DOI: 10.1007/S40071-017-0175-0
关键词: Messenger RNA 、 Open reading frame 、 Protein sequencing 、 Homology (biology) 、 Gene expression 、 Biochemistry 、 Molecular biology 、 Coding region 、 Biology 、 Peroxiredoxin 、 Molecular mass
摘要: The aim of this work was to investigate the function peroxiredoxin family member Peroxiredoxin 6 in Sepiella maindroni (Smprx6) during embryonic development and response thermal stress. 1064 bp full-length coding sequence Smprx6 includes an open reading frame 660 bp encoding a polypeptide 219 amino acids with predicted molecular mass 24.4 kDa. deduced protein shares significant homology other reported Prx6 enzymes. Phylogenetic analysis classified SmPrx6 into 1-Cys-Prx cluster, quantitative real-time PCR (qPCR) demonstrated constitutive mRNA expression all tested tissues five different developmental stages, as previously documented cephalopods. Thermal stress induced expression, which peaked at 6 h then returned control levels. Recombinant active concentrations thiol-dependant. results suggest that plays major role its oxidative
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