Cardioprotective effect of hydroxyfasudil as a specific Rho-kinase inhibitor, on ischemia-reperfusion injury in canine coronary microvessels in vivo.
作者： Fumihiko Kajiya , Hiroaki Shimokawa , Toyotaka Yada
DOI:
关键词: Myosin light-chain kinase 、 Rho kinase inhibitor 、 Reperfusion injury 、 Vasodilation 、 Acetylcholine 、 Myocardial infarction 、 Papaverine 、 Ischemia 、 Cardiology 、 Pharmacology 、 Internal medicine 、 Medicine
摘要: Rho-kinase modulates calcium sensitivity of the myosin light chain in smooth muscle cells and has been implicated as playing a pathogenetic role cardiovascular disorders. This paper was aimed to determine whether hydroxyfasudil (a specific inhibitor) exerts cardioprotective effect on coronary ischemia-reperfusion (I/R) injury, if so, NO is involved. Canine subepicardial small arteries (diameter > or = 100 microm) arterioles < were observed by CCD intravital microscope during I/R. Coronary vascular responses endothelium-dependent (acetylcholine) -independent (papaverine) vasodilators examined after I/R under three conditions: control, preconditioning, hydroxyfasudil. significantly impaired vasodilation acetylcholine, whereas completely preserved responses, did preconditioning. Hydroxyfasudil also reduced myocardial infarct size. These results indicated that effects injury vivo, for which NO-mediated mechanism may be
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