DNA probes for the detection of plant pathogenic bacteria

作者: Ole F. Rasmussen , James C. Reeves

DOI: 10.1016/0168-1656(92)90156-4

关键词: Computational biologyPolymerase chain reactionMolecular probeHybridization probePathogenic bacteriaMicrobiologyDNABacteriaBiologyIdentification (biology)Pseudomonadaceae

摘要: Abstract During the last few years there has been an increasing interest in exploiting potential of DNA probes for identification and detection phytopathogenic bacteria are now sufficient reports using representatives main relevant genera to show that unequivocal value identification. Detection is required under two sets circumstances: diagnosis diseased plant material hygiene purposes. The major difference demand sensitivity thus different probe assays relevance conditions. Dot-blot simple but not very sensitive. On other hand, based on polymerase chain reaction can be extremely A common problem many applications appears a negative influence from debris and/or background microflora. There are, however, several possibilities counteract these effects. In this review, we discuss recent progress made field.

参考文章(45)
B. Steinbrenner, W. Zeller, P. Bellemann, K. Geider, DNA-hybridization, a specific method for the diagnosis of fire blight. Acta Horticulturae. pp. 91- 93 ,(1990) , 10.17660/ACTAHORTIC.1990.273.11
John L. Norelli, Thomas J. Burr, Adriana M. Lo Cicero, Mathew T. Gilbert, Barbara H. Katz, Homologous Streptomycin Resistance Gene Present among Diverse Gram-Negative Bacteria in New York State Apple Orchards. Applied and Environmental Microbiology. ,vol. 57, pp. 486- 491 ,(1991) , 10.1128/AEM.57.2.486-491.1991
Hildegard Falkenstein, Peter Bellemann, Sabine Walter, Wolfgang Zeller, Klaus Geider, Identification of Erwinia amylovora, the Fireblight Pathogen, by Colony Hybridization with DNA from Plasmid pEA29 Applied and Environmental Microbiology. ,vol. 54, pp. 2798- 2802 ,(1988) , 10.1128/AEM.54.11.2798-2802.1988
P L Xu, S Leong, L Sequeira, Molecular cloning of genes that specify virulence in Pseudomonas solanacearum. Journal of Bacteriology. ,vol. 170, pp. 617- 622 ,(1988) , 10.1128/JB.170.2.617-622.1988
P. H. Goodwin, B. C. Kirkpatrick, J. M. Duniway, Identification of Phytophthora citrophthora with Cloned DNA Probes. Applied and Environmental Microbiology. ,vol. 56, pp. 669- 674 ,(1990) , 10.1128/AEM.56.3.669-674.1990
D. A. Cuppels, R. A. Moore, V. L. Morris, Construction and Use of a Nonradioactive DNA Hybridization Probe for Detection of Pseudomonas syringae pv. Tomato on Tomato Plants. Applied and Environmental Microbiology. ,vol. 56, pp. 1743- 1749 ,(1990) , 10.1128/AEM.56.6.1743-1749.1990
A R Datta, B A Wentz, D Shook, M W Trucksess, Synthetic oligodeoxyribonucleotide probes for detection of Listeria monocytogenes. Applied and Environmental Microbiology. ,vol. 54, pp. 2933- 2937 ,(1988) , 10.1128/AEM.54.12.2933-2937.1988