作者: Ole F. Rasmussen , James C. Reeves
DOI: 10.1016/0168-1656(92)90156-4
关键词: Computational biology 、 Polymerase chain reaction 、 Molecular probe 、 Hybridization probe 、 Pathogenic bacteria 、 Microbiology 、 DNA 、 Bacteria 、 Biology 、 Identification (biology) 、 Pseudomonadaceae
摘要: Abstract During the last few years there has been an increasing interest in exploiting potential of DNA probes for identification and detection phytopathogenic bacteria are now sufficient reports using representatives main relevant genera to show that unequivocal value identification. Detection is required under two sets circumstances: diagnosis diseased plant material hygiene purposes. The major difference demand sensitivity thus different probe assays relevance conditions. Dot-blot simple but not very sensitive. On other hand, based on polymerase chain reaction can be extremely A common problem many applications appears a negative influence from debris and/or background microflora. There are, however, several possibilities counteract these effects. In this review, we discuss recent progress made field.