Propagation and Separation of Charged Colloids by Cylindrical Passivated Gel Electrophoresis

摘要: We explore the electrophoretic propagation of charged colloidal objects, monodisperse anionically stabilized polystyrene spheres, in large-pore agarose gels that have been passivated using polyethylene glycol (PEG) when a radial electric field is applied cylindrical geometry. By contrast to standard Cartesian gel-electrophoresis geometries, geometry, particles start at ring well near central axis propagate outward more rapidly initially and then slow down as they move further away from axis. building full-ring gel electrophoresis chamber taking movies scattered light propagating nanospheres undergoing electrophoresis, we experimentally demonstrate ring-like front propagates stably PEG-passivated measured radius function time agrees with simple model incorporates Moreover, show this geometry offers potential advantage performing separations objects widely different sizes: smaller can still be retained has limited size over long run times required for separating larger objects.