Lipid Analysis: Isolation, Separation, Identification and Lipidomic Analysis

摘要: Part 1 introducing lipids and their analysis: Lipids: Their structures occurrence Chromatographic analysis of lipids: general principles Lipid extraction, storage sample handling. 2 chromatographic separation individual lipid classes: Analysis simple classes phospholipids glycosyldiacylglycerols sphingolipids. 3 fatty acids: Preparation derivatives acids Gas acid Isolation identification by spectroscopic related techniques. 4 molecular species Molecular triacylglycerols, diacylglycerols derived from complex lipids, intact glycolipids Positional distributions in glycerolipids. 5 mass spectrometric lipidomics: Introduction to lipidomics Characterization electrospray ionization spectrometry Practical extracts biological samples Quantification spectrometry.