Purification of two distinct growth factors from bovine neural tissue by heparin affinity chromatography.

摘要: Two growth factors have been purified to homogeneity from either bovine hypothalamus or brain by heparin affinity chromatography. Both stimulate the of murine 3T3 fibroblasts and capillary endothelial cells. One heparin-binding factor (HGF alpha), tissue elution with 1.0 M sodium chloride, is obtained in a yield 0.4 mg/kg tissue. Its apparent molecular weight 16 000 dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), its amino acid composition identical that acidic fibroblast recently isolated multistep chromatographic procedure [Thomas, K. A., Rios-Candelore, M., & Fitzpatrick M. (1984) Proc. Natl. Acad. Sci. U.S.A. 81, 357-361]. A second beta), 2.0 0.02 18 SDS-PAGE, differs HGF alpha. These results confirm existence two distinct neural establish cell are identical.