Charge Separation is Virtually Irreversible in Photosystem II Core Complexes with Oxidized Primary Quinone Acceptor
作者： C. D. van der Weij−de Wit , J. P. Dekker , R. van Grondelle , I. H. M. van Stokkum
DOI: 10.1021/JP1083746
关键词: Antenna (radio) 、 Kinetic energy 、 Kinetics 、 Photosystem II 、 Photosynthetic reaction centre 、 Fluorescence 、 Irreversible process 、 Analytical chemistry 、 Chemistry 、 Primary charge separation
摘要: X-ray structures of the Photosystem II (PSII) core revealed relatively large interpigment distances between CP43 and CP47 antenna complexes reaction center (RC) with respect to in a single unit. This finding questions possibility fast energy equilibration among RC, which has been basic explanation for measured PSII fluorescence kinetics more than two decades. In this study, we present time-resolved measurements obtained streak-camera setup on from Thermosynechococcus elongatus at room temperature (RT) 77 K. Kinetic modeling RT data oxidized quinone acceptor Q(A), reveals that are best described by primary charge separation time scale 1.5 ps slow transfer into results an RC about 44 ps. model is consistent structure-based computations. Primary radical pair formation was found be virtually irreversible process. Energy within shown occur 8 K similar scales units as RT, respectively, 7 37 We conclude CP43/CP47 dominant factor total intact cores.
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Alfred R. Holzwarth, Data Analysis of Time-Resolved Measurements Springer, Dordrecht. pp. 75- 92 ,(1996) , 10.1007/0-306-47960-5_5
Ivo H. M. Van Stokkum, Bart Van Oort, Frank Van Mourik, Bas Gobets, Herbert Van Amerongen, (Sub)-Picosecond Spectral Evolution of Fluorescence Studied with a Synchroscan Streak-Camera System and Target Analysis Biophysical Techniques in Photosynthesis, Volume II, Series Advances in Photosynthesis and Respiration. ,vol. 26, pp. 223- 240 ,(2008) , 10.1007/978-1-4020-8250-4_12
Jan P. Dekker, Rienk Van Grondelle, Primary charge separation in Photosystem II. Photosynthesis Research. ,vol. 63, pp. 195- 208 ,(2000) , 10.1023/A:1006468024245
Athina Zouni, Horst-Tobias Witt, Jan Kern, Petra Fromme, Norbert Krauss, Wolfram Saenger, Peter Orth, Crystal structure of photosystem II from Synechococcus elongatus at 3.8 A resolution. Nature. ,vol. 409, pp. 739- 743 ,(2001) , 10.1038/35055589
Marion E. Van Brederode, Michael R. Jones, Frank Van Mourik, Ivo H. M. Van Stokkum, Rienk Van Grondelle, A new pathway for transmembrane electron transfer in photosynthetic reaction centers of Rhodobacter sphaeroides not involving the excited special pair. Biochemistry. ,vol. 36, pp. 6855- 6861 ,(1997) , 10.1021/BI9703756
Grzegorz Raszewski, Thomas Renger, Light harvesting in photosystem II core complexes is limited by the transfer to the trap: can the core complex turn into a photoprotective mode? Journal of the American Chemical Society. ,vol. 130, pp. 4431- 4446 ,(2008) , 10.1021/JA7099826
Marie Louise Groot, Jacques Breton, Luuk J. G. W. van Wilderen, Jan P. Dekker, Rienk van Grondelle, Femtosecond visible/visible and visible/mid-IR pump-probe study of the Photosystem II core antenna complex CP47 Journal of Physical Chemistry B. ,vol. 108, pp. 8001- 8006 ,(2004) , 10.1021/JP037966S
Sergei Vassiliev, Cheng-I Lee, Gary W. Brudvig, Doug Bruce, Structure-based kinetic modeling of excited-state transfer and trapping in histidine-tagged photosystem II core complexes from synechocystis. Biochemistry. ,vol. 41, pp. 12236- 12243 ,(2002) , 10.1021/BI0262597
Frank L. de Weerd, Ivo H.M. van Stokkum, Herbert van Amerongen, Jan P. Dekker, Rienk van Grondelle, Pathways for Energy Transfer in the Core Light-Harvesting Complexes CP43 and CP47 of Photosystem II Biophysical Journal. ,vol. 82, pp. 1586- 1597 ,(2002) , 10.1016/S0006-3495(02)75510-0
Rienk van Grondelle, Jan P Dekker, Tomas Gillbro, Villy Sundstrom, None, Energy transfer and trapping in photosynthesis Biochimica et Biophysica Acta. ,vol. 1187, pp. 1- 65 ,(1994) , 10.1016/0005-2728(94)90166-X