Molecular characterization of a venom acid phosphatase from the Asiatic honeybee Apis cerana
作者： Bo Yeon Kim , Byung Rae Jin
DOI: 10.1016/J.ASPEN.2016.07.013
关键词: Enzyme 、 Biology 、 Acid phosphatase 、 Apis cerana 、 Western blot 、 Phosphatase 、 Recombinant DNA 、 Biochemistry 、 Antibody 、 Venom
摘要: Abstract Bee venom contains a variety of toxic components, including enzymes, peptides, and biogenic amines. An acid phosphatase Acph-1-like protein has been identified from Asiatic honeybee (Apis cerana) venom. However, no molecular information is currently available for phosphatases A. cerana In this study, an (AcVAP) was identified. The amino sequence analysis the predicted AcVAP revealed high identity with other bee phosphatases. anti-AcVAP antibody produced against recombinant (46-kDa) expressed in baculovirus-infected insect cells. Northern Western blot analyses showed that gland present as 46-kDa secreted enzymatic properties were determined using p-nitrophenyl phosphate (p-NPP) substrate exhibited highest activity at pH 4.8 45 °C. Taken together, our data demonstrated functions phosphatase.
sci-hub.se 参考文章(28)
Yeon Ho Je, Jin Hee Chang, Jae Young Choi, Jong Yul Roh, Byung Rae Jin, David R. O'Reilly, Seok Kwon Kang, A defective viral genome maintained in Escherichia coli for the generation of baculovirus expression vectors Biotechnology Letters. ,vol. 23, pp. 575- 582 ,(2001) , 10.1023/A:1010301404445
Donald R. Hoffman, Allergens in bee venom. III. Identification of allergen B of bee venom as an acid phosphatase. The Journal of Allergy and Clinical Immunology. ,vol. 59, pp. 364- 366 ,(1977) , 10.1016/0091-6749(77)90019-7
Roberto Bonasio, Guojie Zhang, Chaoyang Ye, Navdeep S Mutti, Xiaodong Fang, Nan Qin, Greg Donahue, Pengcheng Yang, Qiye Li, Cai Li, Pei Zhang, Zhiyong Huang, Shelley L Berger, Danny Reinberg, Jun Wang, Jürgen Liebig, None, Genomic Comparison of the Ants Camponotus floridanus and Harpegnathos saltator Science. ,vol. 329, pp. 1068- 1071 ,(2010) , 10.1126/SCIENCE.1192428
Bo Yeon Kim, Kwang Sik Lee, Hu Wan, Feng Ming Zou, Yong Soo Choi, Hyung Joo Yoon, Hyung Wook Kwon, Yeon Ho Je, Byung Rae Jin, Anti-elastolytic activity of a honeybee (Apis cerana) chymotrypsin inhibitor. Biochemical and Biophysical Research Communications. ,vol. 430, pp. 144- 149 ,(2013) , 10.1016/J.BBRC.2012.11.056
M.P. Dani, J.P. Edwards, E.H. Richards, Hydrolase activity in the venom of the pupal endoparasitic wasp, Pimpla hypochondriaca Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology. ,vol. 141, pp. 373- 381 ,(2005) , 10.1016/J.CBPC.2005.04.010
Tiago Elias Heinen, Ana Beatriz Gorini da Veiga, Arthropod venoms and cancer. Toxicon. ,vol. 57, pp. 497- 511 ,(2011) , 10.1016/J.TOXICON.2011.01.002
T GRUNWALD, B BOCKISCH, E SPILLNER, J RING, R BREDEHORST, M OLLERT, Molecular cloning and expression in insect cells of honeybee venom allergen acid phosphatase (Api m 3). The Journal of Allergy and Clinical Immunology. ,vol. 117, pp. 848- 854 ,(2006) , 10.1016/J.JACI.2005.12.1331
E. Barboni, D.M. Kemeny, S. Campos, C.A. Vernon, The purification of acid phosphatase from honey bee venom (Apis mellifica) Toxicon. ,vol. 25, pp. 1097- 1103 ,(1987) , 10.1016/0041-0101(87)90266-2
Mary E. Heavner, Gwenaelle Gueguen, Roma Rajwani, Pedro E. Pagan, Chiyedza Small, Shubha Govind, Partial venom gland transcriptome of a Drosophila parasitoid wasp, Leptopilina heterotoma, reveals novel and shared bioactive profiles with stinging Hymenoptera. Gene. ,vol. 526, pp. 195- 204 ,(2013) , 10.1016/J.GENE.2013.04.080
Guntima Suwannapong, Paitoon Seanbualuang, Sivaram Venkatarame Gowda, Mark Eric Benbow, Detection of odor perception in Asiatic honeybee (Apis cerana Fabricius, 1793) workers by changing membrane potential of the antennal sensilla Journal of Asia-pacific Entomology. ,vol. 13, pp. 197- 200 ,(2010) , 10.1016/J.ASPEN.2010.03.001