Interactions of human blood plasma with hydrogen peroxide and hypochlorous acid

摘要: Activated neutrophils produce both hydrogen peroxide (H2O2) and hypochlorous acid (HOCl). Previous work has shown that HOCl depletes antioxidants, modifies proteins, forms fatty chlorohydrins but does not cause significant lipid peroxidation in human plasma. Because activated phagocytes have been claimed to stimulate plasma, we examined the effects of H2O2 alone combination on plasma constituents. Hydrogen at concentrations below 0.5 mmol/L had little effect, 1 2 caused loss ascorbic protein thiol groups, an effect potentiated by preincubation with sodium azide inhibit catalase. no detectable or alpha-tocopherol some depletion ubiquinol occurred. The more than either agent alone. Peroxidation was inhibited metal chelators ethylenediaminetetraacetic deferoxamine singlet O2/hydroxyl radical scavenger histidine. We hypothesize phagocyte-derived could interact microenvironment leukocyte induce lipoproteins cell membranes (or both).