Approaches for the Identification and Characterization of RNA-Protein Interactions
作者： Saiprasad Palusa , Jeffrey Wilusz
DOI: 10.1007/978-1-4614-3991-2_10
关键词: Streptavidin-agarose 、 Computational biology 、 Identification (biology) 、 RNA-binding protein 、 RNA-Binding Motif 、 Characterization (materials science) 、 Regulation of gene expression 、 Computer science 、 Rna protein
摘要: Based on analyses of conventional RNA binding motifs, there are well over 550 proteins produced in living cells. Thus a detailed understanding the mechanisms post-transcriptional regulation requires an how these trans-acting factors act both local and combinatorial fashions. The goal this chapter is to provide roadmap for approaching identification RNA–protein interactions decipher fundamental aspects their roles gene expression. Many techniques outlined below applicable study RNA–RNA as well. Collectively they should rational approach gain mechanistic insights into variety processes.
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