Salt-specific regulation of the cytosolic O-acetylserine(thiol)lyase gene from Arabidopsis thaliana is dependent on abscisic acid.

作者: Consuelo Barroso , Luis C. Romero , Francisco J. Cejudo , José M. Vega , Cecilia Gotor

DOI: 10.1023/A:1006285016296

关键词: O-AcetylserineBiochemistryBiologyCysteineLyase GeneGlutathioneArabidopsisLyaseArabidopsis thalianaAbscisic acid

摘要: The expression of Atcys-3A gene coding for cytosolic O-acetylserine(thiol)lyase, a key enzyme in cysteine biosynthesis, from Arabidopsis thaliana is significantly induced by exposure to salt and heavy-metal stresses. Addition NaCl mature plants rapid accumulation the mRNA throughout leaf lamina roots, later on stems, being mainly restricted vascular tissues. salt-specific regulation was also mediated abscisic acid (ABA) since: (1) exogenous addition ABA culture medium mimicked salt-induced plant response raising level transcript, (2) mutants aba-1 abi2-1 were not able respond NaCl. Our results suggest that high rate biosynthesis required under stress necessary protection or adaptation mechanism. This hypothesis supported observation intracellular levels glutathione increased up 3-fold after treatment.

参考文章(22)
H.U. Gläser, D. Thomas, R. Gaxiola, F. Montrichard, Y. Surdin-Kerjan, R. Serrano, Salt tolerance and methionine biosynthesis in Saccharomyces cerevisiae involve a putative phosphatase gene. The EMBO Journal. ,vol. 12, pp. 3105- 3110 ,(1993) , 10.1002/J.1460-2075.1993.TB05979.X
Cecilia Gotor, Francisco J Cejudo, Consuelo Barroso, José M Vega, Tissue-specific expression of ATCYS-3A, a gene encoding the cytosolic isoform of O-acetylserine(thiol)lyase in Arabidopsis. Plant Journal. ,vol. 11, pp. 347- 352 ,(1997) , 10.1046/J.1365-313X.1997.11020347.X
Meinhart H. Zenk, Heavy metal detoxification in higher plants: a review Gene. ,vol. 179, pp. 21- 30 ,(1996) , 10.1016/S0378-1119(96)00422-2
M. Koornneef, G. Reuling, C. M. Karssen, The isolation and characterization of abscisic acid-insensitive mutants of Arabidopsis thaliana Physiologia Plantarum. ,vol. 61, pp. 377- 383 ,(1984) , 10.1111/J.1399-3054.1984.TB06343.X
Rüdiger Hell, Christiane Bork, Natascha Bogdanova, Igor Frolov, Rüdiger Hauschild, Isolation and characterization of two cDNAs encoding for compartment specific isoforms of O-acetylserine (thiol) lyase from Arabidopsis thaliana FEBS Letters. ,vol. 351, pp. 257- 262 ,(1994) , 10.1016/0014-5793(94)00872-8
Margarita Garcıa-Hernández, Angus Murphy, Lincoln Taiz, None, Metallothioneins 1 and 2 Have Distinct but Overlapping Expression Patterns in Arabidopsis Plant Physiology. ,vol. 118, pp. 387- 397 ,(1998) , 10.1104/PP.118.2.387
C. D. Rock, J. A. Zeevaart, The aba mutant of Arabidopsis thaliana is impaired in epoxy-carotenoid biosynthesis Proceedings of the National Academy of Sciences of the United States of America. ,vol. 88, pp. 7496- 7499 ,(1991) , 10.1073/PNAS.88.17.7496