The use of phospholipid fatty acid analysis to measure impact of acid rock drainage on microbial communities in sediments.

摘要: The impact of acid rock drainage (ARD) and eutrophication on microbial communities in stream sediments above below an abandoned mine site the Adelaide Hills, South Australia, was quantified by PLFA analysis. Multivariate analysis water quality parameters, including anions, soluble heavy metals, pH, conductivity, as well total extractable metal concentrations sediments, produced clustering sample sites into three distinct groups. These groups corresponded with levels nutrient enrichment and/or concentration pollutants associated ARD. Total concentration, which is indicative biomass, reduced >70% at along between far 18 km downstream. Further downstream, however, recovery abundance apparent, possibly reflecting dilution effect downstream tributaries. >40% higher at, immediately below, (0–0.1 km), compared further (2.5–18 even after accounting for differences specific surface area different sediment samples. increased population proximity source may be presence a thriving iron-oxidizing bacteria community consequence optimal conditions these organisms while lower greater sediments’ concentrations. PCA relative revealed number PLFAs were most influential discriminating ARD-polluted rest sites. included hydroxy fatty acids: 2OH12:0, 3OH12:0, 2OH16:0; fungal marker: 18:2ω6; sulfate-reducing marker 10Me16:1ω7; saturated acids 12:0, 16:0, 18:0. Partial constrained ordination that environmental parameters greatest bearing profiles aluminum, iron, zinc. study demonstrated successful application to rapidly assess toxicity ARD-affected waters differentiate this response from effects other pollutants, such nutrients salinity.