Oxidative stress and intracellular pH in the young and old erythrocytes of rat

摘要: The effects of oxidative stress (OS) on the rat erythrocytes (RBCs) that were fractionated percoll/BSA gradient into young and old cells studied to find out if altered Na + /H+ Cl−/HCO3 − antiporters in turn intracellular pH (pHi) could act as one promoters cell death. Old more spherical with lesser surface area, fragile osmotically had protein sulphydryl content than cells. OS was induced RBCs by 2,2′-azobis (2-amidinopropane) dihydrochloride (AAPH). AAPH increased superoxide dismutase (SOD) activity MDA level and, changes between old. Interestingly, vitamin C effective reducing Further, a rapid Na+-dependent acidification presence marginal acidosis evident. exhibited higher less lowering stress-induced compared young. Our studies suggest followed low be determinant factors for disappearance from circulation, perhaps too under OS.