Coimmobilization of Dehydrogenases and Their Cofactors in Electrochemical Biosensors
作者： Maogen Zhang , Conor Mullens , Waldemar Gorski
DOI: 10.1021/AC061698N
关键词: Combinatorial chemistry 、 Biosensor 、 Chemistry 、 NAD+ kinase 、 Cofactor 、 Glucose dehydrogenase 、 Nicotinamide adenine dinucleotide 、 Organic chemistry 、 Nafion 、 Glassy carbon 、 Dehydrogenase
摘要: Enzyme-based reagentless biosensors were developed using the model system of glucose dehydrogenase (GDH) and its nicotinamide adenine dinucleotide cofactor (NAD+). The prepared following an approach similar to concept molecular imprinting. To this end, N1-carboxymethyl−NAD+ species covalently attached polyamino−saccharide chains chitosan (CHIT) allowed interact with GDH in aqueous solution. bioaffinity interactions between NAD+ secured by cross-linking glutaric dialdehyde (GDI)-modified CHIT. Electron conductive films such CHIT−NAD+−GDH−GDI−CHIT macrocomplexes (MC) on glassy carbon (GC) electrodes adding nanotubes (CNT) evaporating water. Electrochemical analysis GC/CNT−MC revealed that, contrast oxidase-based electrodes, they acted as oxygen-independent biosensors. application Nafion predictably improved their selectivity...
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