In vivo Tube Assay: An Optimised Protocol of the Directed in vivo Angiogenesis Assay by Implementing Immunohistochemistry.
作者： Matthias Unseld , Johannes M. Breuss , Clemens Pausz , Eleonore Pablik , Gernot Schabbauer
DOI: 10.1159/000434751
关键词: Biomedical engineering 、 Ex vivo 、 In vivo 、 Angiogenesis 、 Biology 、 Angiogenesis Inducing Agents 、 Fibroblast growth factor 、 Neovascularization 、 Pathology 、 CD31 、 Vascular endothelial growth factor A
摘要: Background: Angiogenesis, the formation of new blood vessels, is an essential process under physiological and pathological conditions. Method: Here, we improved directed in vivo angiogenesis assay (DIVAA®) test, which based on usage small Matrigel-filled tubes that are implanted into mice subcutaneously for a period up to 15 days. The subsequent ex assessment neoangiogenesis within silicon then achieved by fluorometry. Results: We showed immunohistochemical quantification ingrowth endothelial cells, CD31, was superior fluorometric advised manufacturer's instructions. optimised explantation procedure, ensuring complete recovery ingrown vessels. Using this modified protocol, investigated effect length stay as well concentration growth factors VEGF FGF assay. Conclusion: Our protocol offered effective reliable alternative original assay, expected facilitate research and, thus, might drive development novel therapeutic agents.
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