Highly porous 3D nanofibrous scaffolds processed with an electrospinning/laser process

摘要: Abstract Electrospinning has been widely used to produce micro/nanosized fibres. Although the method is very simple, easy, and effective for obtaining nanosized material, fabrication of three dimensional (3D) shapes comprised micro/nanofibres a major obstacle use in tissue engineering. In this study, new electrospinning fabricate controllable 3D micro/nanofibrous structure (with thickness over 3 mm) suggested. The fabricated fibrous was fully porous successfully consisted submicron-sized However, pores were too small (5–10 μm), so we femtosecond laser process achieve enough cell infiltration proliferation direction structure. By controlling appropriate processing conditions, can highly with various pore sizes ranging from 189 ± 28 μm 380 ± 21 μm. scaffolds assessed in vitro biological capabilities by culturing osteoblast like cells (MG63). Compared rapid-prototyped PCL scaffold, scaffold exhibited significantly higher activities (initial attachment proliferation) due topographical micro/nanofibres.