Filters and mirrors for applications in fluorescence microscopy.

摘要: Publisher Summary This chapter describes the filters and mirrors for applications in fluorescence microscopy. The serves as a short summary of fluorescence; and, specifically, required some current methods One most prominent changes microscopy is recent explosion associated with Fluorescence phenomenon which substance (atom or molecule) absorbs light specific wavelength, rapidly radiates another (usually longer) wavelength. substances that exhibit properties are called fluorescent molecules, fluorochromes, fluorophores. absorption initial photon termed “excitation,” functions to move fluorophore from ground state an excited state. From this intermediate, singlet state, there three principal options available: (1) intersystem crossing, can lead prolonged emission phosphorescence; (2) nonradiative conversion; (3) electron falls back