Demonstration of cytomegalovirus nucleic acids in the coronary arteries of transplanted hearts.

摘要: Cytomegalovirus (CMV) infection has been associated with the development of accelerated arteriosclerosis in heart transplant recipients. This association prompted authors to examine, using situ hybridization techniques, coronary arteries 19 patients who had received transplants for presence CMV nucleic acids. Two probes were employed on formalin-fixed paraffin-embedded tissues. The first was an S35-labeled nick translated probe derived from DNA. acids identified arterial intima 5 (45%) 11 transplanted hearts examined this probe. Hybridization cells morphologically consistent endothelial cells, lymphocytes, and smooth muscle identified. not detected any when a control probe, vector without DNA, employed. second that used tritium-labeled strand-specific ribo-probe immediate early (IE1) gene CMV. Using three five positive DNA one negative (38%) eight additional hearts. pattern same as seen no sense IE1 These results suggest are present