In vitro and in vivo antitrypanosomal efficacy of combination therapy of Anogeissus leiocarpus, Khaya senegalensis and potash.

摘要: Abstract Ethnopharmacological relevance Pastoralists in Nigeria mix barks of Anogeissus leiocarpus (AL) Khaya senegalensis (KS) and potash (Pt) to treat animal African trypanosomosis. Aim To evaluate antitrypanosomal potential A. leiocarpus, K. for insights into the traditional claim combination therapy (ATCT). Materials methods Fifty microliter each six different concentrations AL, KS, Pt, AL + KS, AL + KS + Pt diminazene aceturate (DA, positive control) was incubated with 50 μL parasite-laden blood containing 108 Trypanosoma congolense cells a 96-well microtitre plate. Negative control wells were devoid extracts drug but supplemented phosphate-buffered saline (PBS). Efficacy treatment observed at 1 h interval complete immobilisation or reduced motility parasites. Each mixture inoculated mouse point parasite end 6-h observation period that did not immobilise parasites completely. For vivo assessment, thirty-five parasitaemic rats randomly allocated seven groups 5 each. rat I–V treated 500 mg/kg AL + KS AL + KS + Pt, respectively, 7 days. Rats VI VII 3.5 mg/kg once PBS 2 mL/kg (7 days), which served as negative controls, respectively. Daily monitoring parasitaemia through tail vein, packed cell volume malondialdehyde used assess efficacy treatments. Results The group significantly (p  Conclusion showed better effects than single offers prospects ATCT. Our findings support ethnopharmacological use combined by pastoralist trypanosomosis Nigeria.