Non-Invasive In Vivo Imaging and Quantification of Tumor Growth and Metastasis in Rats Using Cells Expressing Far-Red Fluorescence Protein

摘要: Non-invasive in vivo imaging is emerging as an important tool for basic and preclinical research. Near-infrared (NIR) fluorescence dyes probes have been used non-invasive optical since the NIR region absorption auto by body tissue low, thus permitting greater penetration depths high signal to noise ratio. Currently, cell tracking systems rely on labeling cells prior injection or administering targeting population of choice right before imaging. These approaches do not enable tumor growth, label diluted during division. In this study we developed lines stably expressing far-red protein E2-Crimson, enabling continuous detection quantification growth. a xenograft rat model, show that E2-Crimson can be detected over 5 week period using Fluorescence intensities correlated with volume weight allowed reliable robust entire compartment. Using novel regime, seeding MDA-MB-231 breast cancer lungs model was established verified.