Specific activity staining for prostaglandin metabolizing enzymes on polyacrylamide gel.
作者： Tetsuji Tanaka , Norimasa Mori
DOI: 10.1016/0262-1746(86)90194-0
关键词: Biochemistry 、 Staining 、 Prostaglandin 、 Dehydrogenase 、 Specific activity 、 Digestion 、 Gel electrophoresis 、 Polyacrylamide gel electrophoresis 、 Molecular biology 、 Chemistry 、 Enzyme
摘要: A simple activity staining technique on polyacrylamide gel for 15-hydroxyprostaglandin metabolizing enzymes was developed in which production or digestion of 15-keto-PGE stained. The rapidity and specificity this shown with a preparation NAD-linked dehydrogenase isolated from human term placenta.
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