Plasmid maintenance in Bacillus stearothermophilus is strain-dependent.
作者： Sierd Bron , Linda Oskam , Gerard Venema
DOI: 10.1016/0378-1097(92)90463-X
关键词: Molecular cloning 、 Replicon 、 Rolling circle replication 、 Plasmid 、 Plasmid preparation 、 Strain (chemistry) 、 Bacillaceae 、 Plasmid maintenance 、 Biology 、 Molecular biology
摘要: We studied the segregational stability of plasmids based on pTB913, a 4.5-kb rolling-circle plasmid derived from thermophilic Bacillus pTB19. In stearothermophilus pTB913 derivatives appeared to be strain-dependent. strain CU21 large amounts single-stranded DNA were found and was highly unstable at 57°C. NUB3621, however, very low formed pTB913-based replicons only slightly The NUB3621/pTB913 host-vector system seems appropriate for molecular cloning. A RepA-based replicon, also pTB19 but replicating by theta mechanism, in B. NUB3621.
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