Molecular characterization of Thai Salmonella enterica serotype typhimurium and serotype 4,5,12:i:- reveals distinct genetic deletion patterns
作者： Laingshun Huoy , Srirat Pornruangwong , Chaiwat Pulsrikarn , Soraya Chaturongakul
关键词: Serotype 、 Pulsed-field gel electrophoresis 、 Genetic marker 、 Polymerase chain reaction 、 Subtyping 、 Tetracycline 、 Biology 、 Salmonella enterica 、 Microbiology 、 Salmonella
摘要: In order to better understand the relationship between Salmonella serotypes Typhimurium and its monophasic variant 4,5,12:i:- found in Thailand, a total of 138 isolates from various sources were characterized using different molecular subtyping methods (i.e., pulsed-field gel electrophoresis [PFGE] polymerase chain reaction [PCR]) antibiotic resistance (AbR) patterns. PFGE revealed 52 distinct band patterns among these isolates, 3 which shared two serotypes. PCR characterization genomic deletion reveals that Thai S. contain pattern fljAB region, can be used as specific genetic marker for primary identification sources. AbR study shows that, 50 representative serotype-confirmed strains, 48.28% (14/29) 90.48% (19/21) are multidrug-resistant they resistant at least antimicrobial categories. The AmpST ampicillin, streptomycin, tetracycline was high proportions (10 29) (15 21) isolates.
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