Lnc-PICSAR contributes to cisplatin resistance by miR-485-5p/REV3L axis in cutaneous squamous cell carcinoma.
作者： Dan Wang , Xiaoqiang Zhou , Jing Yin , Yang Zhou
关键词: REV3L 、 Cisplatin 、 Gene silencing 、 Biology 、 Cell migration 、 Cancer research 、 Gene knockdown 、 Exosome 、 Cell 、 Viability assay
摘要: Background Dysregulation of long noncoding RNAs (lncRNAs) is associated with drug resistance in multiple cancers. We explored the roles lncRNA p38 inhibited cutaneous squamous cell carcinoma-associated lincRNA (PICSAR) cisplatin (DDP) carcinoma (CSCC). Methods Quantitative real-time polymerase chain reaction (qRT-PCR) was employed to measure expression lnc-PICSAR, miR-485-5p and reversionless 3-like (REV3L) mRNA. The counting kit-8 (CCK-8) assay conducted evaluate DDP viability. transwell performed determine migration invasion. Western blot immunohistochemistry (IHC) staining were carried out protein levels. dual-luciferase reporter used investigate association between lnc-PICSAR or REV3L. Murine xenograft model constructed explore function vivo. morphology exosomes analyzed by transmission electron microscopy (TEM) nanoparticle tracking analysis (NTA). Results Lnc-PICSAR elevated DDP-resistant CSCC cells. silencing suppressed viability, resistance, invasion MiR-485-5p acted as a target inhibition reversed impacts on progression promoted REV3L via sponging miR-485-5p. Moreover, overexpression overturned effects resistance. knockdown In addition, increased derived from patients' serum Conclusion enhanced miR-485-5p/REV3L axis Besides, exosome-mediated might be involved regulation CSCC.
sci-hub.st 参考文章(29)
Jean-Pierre Gillet, Michael M. Gottesman, Mechanisms of Multidrug Resistance in Cancer Methods in Molecular Biology. ,vol. 596, pp. 47- 76 ,(2010) , 10.1007/978-1-60761-416-6_4
WENJIE WANG, WENJIONG SHENG, CHENXIAO YU, JIANPING CAO, JUNDONG ZHOU, JINCHANG WU, HUOJUN ZHANG, SHUYU ZHANG, REV3L modulates cisplatin sensitivity of non-small cell lung cancer H1299 cells Oncology Reports. ,vol. 34, pp. 1460- 1468 ,(2015) , 10.3892/OR.2015.4121
Zhihua Li, Xiaohui Zhao, Yu Zhou, Yimin Liu, Quanbo Zhou, Huilin Ye, YinXue Wang, Jinlong Zeng, Yadong Song, Wenchao Gao, ShangYou Zheng, Baoxiong Zhuang, Huimou Chen, Wenzhu Li, Haigang Li, Haifeng Li, Zhiqiang Fu, Rufu Chen, The long non-coding RNA HOTTIP promotes progression and gemcitabine resistance by regulating HOXA13 in pancreatic cancer. Journal of Translational Medicine. ,vol. 13, pp. 84- 84 ,(2015) , 10.1186/S12967-015-0442-Z
Rituparno Sen, Suman Ghosal, Shaoli Das, Subrata Balti, Jayprokas Chakrabarti, Competing Endogenous RNA: The Key to Posttranscriptional Regulation The Scientific World Journal. ,vol. 2014, pp. 896206- 896206 ,(2014) , 10.1155/2014/896206
Ning Xu, Lingyun Zhang, Florian Meisgen, Masako Harada, Johan Heilborn, Bernhard Homey, Dan Grandér, Mona Ståhle, Enikö Sonkoly, Andor Pivarcsi, MicroRNA-125b Down-regulates Matrix Metallopeptidase 13 and Inhibits Cutaneous Squamous Cell Carcinoma Cell Proliferation, Migration, and Invasion Journal of Biological Chemistry. ,vol. 287, pp. 29899- 29908 ,(2012) , 10.1074/JBC.M112.391243
Murad Alam, Désirée Ratner, Cutaneous Squamous-Cell Carcinoma The New England Journal of Medicine. ,vol. 344, pp. 975- 983 ,(2001) , 10.1056/NEJM200103293441306
Kevin C. Wang, Howard Y. Chang, Molecular Mechanisms of Long Noncoding RNAs Molecular Cell. ,vol. 43, pp. 904- 914 ,(2011) , 10.1016/J.MOLCEL.2011.08.018
P. E. M. Gibbs, W. G. McGregor, V. M. Maher, P. Nisson, C. W. Lawrence, A human homolog of the Saccharomyces cerevisiae REV3 gene, which encodes the catalytic subunit of DNA polymerase ζ Proceedings of the National Academy of Sciences of the United States of America. ,vol. 95, pp. 6876- 6880 ,(1998) , 10.1073/PNAS.95.12.6876
Sofie Claerhout, Lien Verschooten, Sofie Van Kelst, Rita De Vos, Charlotte Proby, Patrizia Agostinis, Marjan Garmyn, Concomitant inhibition of AKT and autophagy is required for efficient cisplatin-induced apoptosis of metastatic skin carcinoma International Journal of Cancer. ,vol. 127, pp. 2790- 2803 ,(2010) , 10.1002/IJC.25300
Kaladhar B Reddy, MicroRNA (miRNA) in cancer Cancer Cell International. ,vol. 15, pp. 38- 38 ,(2015) , 10.1186/S12935-015-0185-1