Specific Protein Patterns Characterize Metastatic Potential of Advanced Bladder Cancer
作者： Rico Pilchowski , Robert Stöhr , Ferdinand von Eggeling , Arndt Hartmann , Heiko Wunderlich
DOI: 10.1016/J.JURO.2011.03.124
关键词: Pathology 、 Cancer 、 Bladder cancer 、 Medicine 、 Tumor progression 、 Laser capture microdissection 、 Urinary bladder 、 Mass spectrometry 、 Metastasis 、 Gel electrophoresis
摘要: Purpose: The prognosis in patients with metastasized bladder cancer is still poor. Clinical and histopathological parameters have limited ability to predict the risk of tumor progression. Thus, we identified specific protein patterns associated progression differentiate specimens without metastasis.Materials Methods: We analyzed 46 42 nonmetastasized muscle invasive cancers by ProteinChip® technology surface enhanced laser desorption/ionization time flight mass spectrometry. Cell lysis was done after capture microdissection from cryostat sections achieve high cell purity. Surface spectrometry completed 2 matrices (Q10 CM10). Bioinformatic analysis performed XLMiner® clustering using Fuzzy c-means method. Differentially expressed proteins were verified 2-dimensional gel electrophoresis, tryptic digest, peptide mapping, immunodepletio...
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