CK2-Dependent Phosphorylation of the Brg1 Chromatin Remodeling Enzyme Occurs during Mitosis
作者： Teresita Padilla-Benavides , Dominic T. Haokip , Yeonsoo Yoon , Pablo Reyes-Gutierrez , Jaime A. Rivera-Pérez
DOI: 10.3390/IJMS21030923
关键词: Casein kinase 2 、 Mitosis 、 Chromatin remodeling 、 Myoblast proliferation 、 Hyperphosphorylation 、 Phosphorylation 、 Kinase 、 Chromatin 、 Cell biology 、 Chemistry
摘要: Brg1 (Brahma-related gene 1) is one of two mutually exclusive ATPases that can act as the catalytic subunit mammalian SWI/SNF (mSWI/SfigureNF) chromatin remodeling enzymes facilitate utilization DNA in eukaryotic cells. a phospho-protein, and its activity regulated by specific kinases phosphatases. Previously, we showed interacts with phosphorylated casein kinase 2 (CK2) manner regulates myoblast proliferation. Here, use biochemical cell molecular biology approaches to demonstrate Brg1-CK2 interaction occurred during mitosis embryonic mouse somites primary myoblasts derived from satellite cells isolated skeletal muscle tissue. The CK2 incorporation number other subunits into mSWI/SNF enzyme complex were independent enzymatic activity. CK2-mediated hyperphosphorylation was observed mitotic multiple types organisms, suggesting functional conservation across tissues species. mitotically hyperphosphorylated form localized soluble chromatin, demonstrating phosphorylation associated partitioning within subcellular compartments. Thus, acts localization.
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