CK2-dependent phosphorylation of the Brg1 chromatin remodeling enzyme occurs during mitosis
作者： Teresita Padilla-Benavides , Dominic T. Haokip , Yeonsoo Yoon , Pablo Reyes-Gutierrez , Jaime A. Rivera-Pérez
DOI: 10.1101/781781
关键词: Cell biology 、 Hyperphosphorylation 、 Myoblast proliferation 、 Mitosis 、 Phosphorylation 、 Chromatin 、 Chemistry 、 Enzyme complex 、 Casein kinase 2 、 Chromatin remodeling
摘要: ABSTRACT Brg1 (Brahma related gene 1) is one of two mutually exclusive ATPases that can act as the catalytic subunit mammalian SWI/SNF chromatin remodeling enzymes facilitate utilization DNA in eukaryotic cells. a phospho-protein and its activity regulated by specific kinases phosphatases. Previously, we showed interacts with phosphorylated casein kinase 2 (CK2) manner regulates myoblast proliferation. Here demonstrate Brg1-CK2 interaction occurred during mitosis embryonic somites primary myoblasts derived from satellite cells isolated muscle tissue. The CK2 incorporation number other subunits into mSWI/SNF enzyme complex were independent enzymatic activity. CK2-mediated hyperphosphorylation was observed mitotic multiple cell types organisms, suggesting functional conservation across tissues species. mitotically hyperphosphorylated form localized soluble chromatin, demonstrating phosphorylation associated partitioning within sub-cellular compartments. Thus acts localization. HIGHLIGHTS Interactions between occur CK2-Brg1 interactions are event conserved
sci-hub.st 参考文章(96)
Jeffrey A. Nickerson, Benjamin J. Blencowe, Sheldon Penman, The Architectural Organization of Nuclear Metabolism International Review of Cytology. ,vol. 162, pp. 67- 123 ,(1996) , 10.1016/S0074-7696(08)61229-2
C. Muchardt, J. C. Reyes, B. Bourachot, E. Leguoy, M. Yaniv, The hbrm and BRG-1 proteins, components of the human SNF/SWI complex, are phosphorylated and excluded from the condensed chromosomes during mitosis. The EMBO Journal. ,vol. 15, pp. 3394- 3402 ,(1996) , 10.1002/J.1460-2075.1996.TB00705.X
Natasha C. Chang, Michael A. Rudnicki, Satellite Cells Current Topics in Developmental Biology. ,vol. 107, pp. 161- 181 ,(2014) , 10.1016/B978-0-12-416022-4.00006-8
P. Lorenz, R. Pepperkok, W. Ansorge, W. Pyerin, Cell biological studies with monoclonal and polyclonal antibodies against human casein kinase II subunit beta demonstrate participation of the kinase in mitogenic signaling. Journal of Biological Chemistry. ,vol. 268, pp. 2733- 2739 ,(1993) , 10.1016/S0021-9258(18)53835-7
Ivana L. de la Serna, Kerri A. Carlson, Anthony N. Imbalzano, Mammalian SWI/SNF complexes promote MyoD-mediated muscle differentiation. Nature Genetics. ,vol. 27, pp. 187- 190 ,(2001) , 10.1038/84826
R. Pepperkok, P. Lorenz, W. Ansorge, W. Pyerin, Casein kinase II is required for transition of G0/G1, early G1, and G1/S phases of the cell cycle. Journal of Biological Chemistry. ,vol. 269, pp. 6986- 6991 ,(1994) , 10.1016/S0021-9258(17)37471-9
Xin Xu, Paul A. Toselli, Lonnie D. Russell, David C. Seldin, Globozoospermia in mice lacking the casein kinase II alpha' catalytic subunit. Nature Genetics. ,vol. 23, pp. 118- 121 ,(1999) , 10.1038/12729
Teresita Padilla-Benavides, Brian T. Nasipak, Anthony N. Imbalzano, Brg1 Controls the Expression of Pax7 to Promote Viability and Proliferation of Mouse Primary Myoblasts Journal of Cellular Physiology. ,vol. 230, pp. 2990- 2997 ,(2015) , 10.1002/JCP.25031
Weidong Wang, Jacques Côté, Yutong Xue, Sharleen Zhou, Paul A Khavari, Stephen R Biggar, Christian Muchardt, Ganjam V Kalpana, Stephen P Goff, Moshe Yaniv, Jerry L Workman, Gerald R Crabtree, Purification and biochemical heterogeneity of the mammalian SWI-SNF complex. The EMBO Journal. ,vol. 15, pp. 5370- 5382 ,(1996) , 10.1002/J.1460-2075.1996.TB00921.X
D. Thrower, M.A. Jordan, L. Wilson, Effects of vinblastine, podophyllotoxin and nocodazole on mitotic spindles. Implications for the role of microtubule dynamics in mitosis. Journal of Cell Science. ,vol. 102, pp. 401- 416 ,(1992) , 10.1242/JCS.102.3.401