Multiple gene expression including sorf constructs and methods with polyproteins, pro-proteins, and proteolysis.

摘要: Disclosed are useful constructs and methods for the expression of proteins using primary translation products that processed within a recombinant host cell. Constructs comprising single open reading frame (sORF) described protein including multiple polypeptides. A product (a pro-protein or polyprotein) contains polypeptides such as inteins hedgehog family auto-processing domains, variants thereof, inserted in between subunits interest. The can also contain cleavage sequences other proteolytic protease recognition sites, signal peptides which peptidases, separating at least two subunits. sites be manipulated to enhance efficiency separate Also disclosed independent aspects conducting efficient expression, secretion, and/or multimeric assembly immunoglobulins. Where polyprotein immunoglobulin heavy light chain segments fragments capable antigen recognition, an embodiment selectable stoichiometric ratio is copies segment per segment, with result production properly folded assembled functional antibody made. Modified peptides, from chains, described.