Single step poly(l-Lysine) microgel synthesis, characterization and biocompatibility tests

摘要: Abstract In this study, an amino acid microgel from l -Lysine as poly( -Lysine) (L1) was prepared in a single step for the first time via microemulsion polymerization/crosslinking technique using tetrakis (hydroxymethyl)phosphium chloride (THPC) crosslinking agent. The spherical L1 microgels with size range of few micrometers revealed by SEM images, were readily modifiable terms surface charges upon simple treatment HCl and NaOH to obtain L2 L3 forms due protonation amine groups deprotonation carboxylic provide tunable charge on synthesized microgels. zeta potential increased to +29.6 mV decreased -41.8 mV -19.1 mV Moreover, biocompatibility tests interestingly showed L3>L2>L1 over 85% up 300 μg/mL particle concentration. Furthermore, blood compatibility that have high hemocompatibility 5.12 ± 0.58% 0.55 ± 0.27% hemolysis ratios, effective hemostatic properties 96.17 ± 2.15 99.63 ± 0.17 clotting indices, respectively; whereas particles found be non-blood compatible their highly positively charged nature. Therefore, possess great may used in vivo biomedical purposes.