A novel gene from Brugia sp. that encodes a cytotoxic fatty acid binding protein allergen recognized by canine monoclonal IgE and serum IgE from infected dogs.
作者： Susan M. Orton , Prema Arasu , Bruce Hammerberg
DOI: 10.1645/GE-1217.1
关键词: Immunoglobulin E 、 Brugia malayi 、 Wuchereria bancrofti 、 Onchocerca volvulus 、 Molecular biology 、 Antigen 、 Expressed sequence tag 、 Brugia pahangi 、 Biology 、 Nucleic acid sequence
摘要: Brugia pahangi infection of dogs is a well characterized model human lymphatic filariasis in which sera consistently show IgG or IgE reactivity to 35-kDa antigen. Using dog lymph node B cells, we previously established heterohybridoma cell line producing canine monoclonal (cmAb 2.39) that activates and degranulates mast specifically recognizes B. By affinity purification sequencing the native protein from adults, 19-amino acid sequence was obtained; derived nucleotide showed homology malayi 2 related Onchocerca volvulus expressed tag (EST) clones Filarial Genome Project database. Consensus primers amplified 244-bp product adult infective larval stage cDNA libraries malayi, O. volvulus, Wuchereria bancrofti, but not those nonfilarial nematodes. The EST clone only sequences. A 684-bp region from...
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