Expression and new exon mutations of the human Beta defensins and their association on colon cancer development.
作者： Abdelhabib Semlali , Abdullah Al Amri , Arezki Azzi , Omair Al Shahrani , Maha Arafah
DOI: 10.1371/JOURNAL.PONE.0126868
关键词: Defensin 、 Exon 、 Gene expression 、 Regulation of gene expression 、 Cancer 、 Beta defensin 、 Biology 、 DNA methylation 、 Mutation 、 Molecular biology
摘要: The development of cancer involves genetic predisposition and a variety environmental exposures. Genome-wide linkage analyses provide evidence for the significant many diseases to susceptibility loci on chromosome 8p23, location human defensin gene cluster. Human β-defensins (hBDs) are important molecules innate immunity. This study was designed analyze expression variations in hBDs (hBD-1, hBD-2, hBD-3 hBD-4) their putative association with colon cancer. hBD relative protein were evaluated by Real-Time polymerase chain reaction (qPCR) immunohistochemistry, respectively, from 40 normal patients age-matched Saudi Arabia. In addition, polymorphisms genotyped exon sequencing promoter methylation. hBD-1, hBD-4 basal messenger RNA significantly lower tumor tissues compared tissues. Several insertion mutations detected different exons analyzed hBDs. However, no methylation any promoters because limited number CpG islands these regions. We demonstrated first time link between suggests that there is immunity deregulation through disruption cationic peptides potential
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