Rotavirus-specific cytotoxic T lymphocytes recognize overlapping epitopes in the amino-terminal region of the VP7 glycoprotein.

摘要: Abstract Rotavirus-specific cytotoxic T lymphocytes (CTL) play an important role in the resolution of rotavirus infection. The outer capsid glycoprotein, VP7, elicits a class I MHC-restricted CTL response. Vaccinia virus recombinants expressing VP7 genes from simian SA11 (serotype G3) and RF strain bovine G6) were used to analyze activity this antigen BALB/c (H-2 d ) C57BL/6 b mice neonatally infected with homologous heterologous rotaviruses. A vaccinia recombinant first amino-terminal 88 amino acids was constructed search for cross-reactive against region protein. By using synthetic K , D motif-fitting peptides two overlapping epitopes have been identified located hydrophobic domain (H1) VP7. Splenocytes obtained SA11-infected induced strongest response target cells sensitized peptide containing -restricted epitope (amino 8–16). second (residues 5–13) recognized by splenocytes derived rotavirus-infected mice. These findings reveal existence H1 signal sequence glycoprotein that coexist 31–40) previously described within H2 region.