Happy Birthday Protein Kinase C: Past, Present and Future of a Superfamily

摘要: Protein Kinase C (PKC) was born in Japan 1977 the Department of Biochemistry University Kobe. Inoue, Kishimoto and Takai Nishizuka’s Laboratory, described two papers Journal Biological Chemistry a cyclic nucleotide-independent, proteolytically modified protein kinase from mammalian brain (named PKM, M for magnesium ions that were indispensable activation) (1,2). The full length enzyme (2), subsequently demonstrated to be activated by calcium phospholipids (3), named (C, ions, which fully at low concentrations, thus differentiate it nucleotide-dependent kinases, A G). same group observed unsaturated diacylglycerol an essential activator PKC (4), linking previously receptor-dependent inositol phospholipid hydrolysis (5) phosphorylation, thrusting this into intercellular signal transduction research. inhibitors characterized 1980 as interfering drugs (such chlorpromazine, imipramine dibucaine) (6) first direct functional assignment made utilizing human platelets thrombin-induced release serotonin shown mediated activation (7). Starting early eighties, interest crossed Japan’s borders invaded rest world, making one most studied enzymes biology, with more than 45.000 research published up now.