High-throughput cytochrome P450 inhibition assays using laser diode thermal desorption-atmospheric pressure chemical ionization-tandem mass spectrometry.

摘要: This paper describes the development of a high-throughput method for analysis cytochrome P450 (CYP) inhibition assay incubation samples using laser diode thermal desorption interfaced with atmospheric pressure chemical ionization mass spectrometry (LDTD-APCI-MS). Data CYP isoforms 3A4, 2D6, 2C9, and 1A2 from competitive assays are shown. The potential was measured by monitoring level metabolites 6β-hydroxytestosterone (3A4), dextrorphan (2D6), 4‘-hydroxydiclofenac (2C9), acetaminophen (1A2) formed in presence drug candidates an eight-point titration. analytical involves plating on specially designed 96-well plates stainless steel bottoms, followed direct LDTD source. Validation LDTD-MS performed testing interferences, reproducibility, dynamic range, ion suppression, ability source to produce comparable results pr...