Effects of Methomyl and Helminthosporium maydis Toxin on Matrix Volume, Proton Motive Force, and NAD Accumulation in Maize (Zea mays L.) Mitochondria.

摘要: Methomyl and Helminthosporium maydis race T toxin block oxidative phosphorylation in mitochondria isolated from maize plants with Texas male sterile cytoplasm (T) but not those Normal (N) (Bednarski, Izawa, Scheffer 1977 Plant Physiol 59: 540-545). Moreover, they have been reported to cause specific swelling (Miller, Koeppe 1971 Science 173: 67-69; Koeppe, Cox, Malone 1978 201: 1227-1229). We could detect, by direct volume measurements, any change induced these compounds the mitochondrial matrix space. show here that proton motive force, which is composed of a large transmembrane potential low pH difference, absent incubated presence methomyl or toxin, while it unchanged N mitochondria. induce, independently collapse release cofactors NAD coenzyme A In particular, we transported mitochondria, this transport, dependent on inhibited toxin.