Stimulation of the antigen and interleukin-2 receptors on T lymphocytes activates distinct tyrosine protein kinases.

摘要: The T cell antigen receptor complex (TCR) and the interleukin 2 (IL-2) are responsible for signal transduction that results in lymphocyte activation proliferation. Stimulation of either TCR or IL-2 induces an increase tyrosine phosphorylation several cellular proteins indicating by both these receptors involves a protein kinase. Although kinases activated have not yet been characterized themselves known to contain kinase domain. To determine if coupled similar distinct we examined patterns kinetics induced stimulation on cloned line. Hut 78.3 cells co-express p75 receptor. These were stimulated with OKT3 antibodies, specific TCR, IL-2. Signal was found set unique each stimulus. antibodies also differed from OKT3-dependent reached maximal levels within 2.5 min began decline 5 after stimulation. In contrast, IL-2-induced did achieve until 15 addition remained phosphorylated even 60 incubation. phosphorylations affected prior other agent. demonstrate different pathways independent kinases.