Structure and Membrane-Targeting Mechanism of Retinal Ca2+-Binding Proteins, Recoverin and GCAP-2
作者： B. Ames , Mitsuhiko Ikura
DOI: 10.1007/978-1-4615-0121-3_20
关键词: Calcium-binding protein 、 Myristoylation 、 Visual phototransduction 、 Chemistry 、 Hippocalcin 、 Protein structure 、 Retinal 、 Cell biology 、 Eye Proteins 、 Recoverin
摘要: Recoverin and the guanylate cyclase activating proteins (GCAPs) are calcium-sensing in retinal rod cone cells that belong to EF-hand superfamily serve as important calcium sensors vision. GCAP myristoylated at their amino-terminus targeted disc membranes by a myristoyl switch. Here, we present three-dimensional, atomic-resolution structures of recombinant recoverin containing 0, 1 2 ions (Ca2+) bound unmyristoylated GCAP-2 with 3 Ca2+bound determined nuclear magnetic resonance. The Ca2+-induced structural changes these for elucidating membrane-targeting mechanisms understanding molecular mechanism Ca2+-sensitive regulation phototransduction.
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