Prime-boost therapeutic vaccination in mice with DNA/DNA or DNA/Fowlpox virus recombinants expressing the Human Papilloma Virus type 16 E6 and E7 mutated proteins fused to the coat protein of Potato virus X.
作者： Elena Illiano , Massimiliano Bissa , Francesca Paolini , Carlo Zanotto , Carlo De Giuli Morghen
DOI: 10.1016/J.VIRUSRES.2016.09.011
关键词: Fowlpox 、 Immune system 、 Blot 、 Immunogenicity 、 Heterologous 、 Potato virus X 、 Virology 、 Fowlpox virus 、 Biology 、 DNA 、 Molecular biology
摘要: The therapeutic antitumor potency of a prime–boost vaccination strategy was explored, based on the mutated, nontransforming forms E6 (E6F47R) and E7 (E7GGG) oncogenes Human Papilloma Virus type 16 (HPV16), fused to Potato virus X (PVX) coat protein (CP) sequence. Previous data showed that CP fusion improves immunogenicity tumor-associated antigens may thus increase their efficacy. After verifying correct expression E6F47RCP E7GGGCP inserted into DNA Fowlpox recombinants by Western blotting immunofluorescence, combined use evaluated for therapy in pre-clinical mouse model HPV16-related tumorigenicity. Immunization protocols were applied using homologous (DNA/DNA) or heterologous (DNA/Fowlpox) vaccine regimens. humoral immune responses determined ELISA, efficacy delay tumor appearance reduced volume after inoculation syngeneic TC-1* cells. Homologous DNA/DNA genetic vaccines able better inhibit growth when DNAE6F47RCP DNAE7GGGCP administered combination. However, DNA/Fowlpox higher number animals particular alone.
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