Isolation and characterization of four microsatellite loci in the tidewater goby (Eucyclogobius newberryi).
作者： Holly Mendonca , Jerry Smith , Chris Brinegar
关键词: Microsatellite 、 Allele 、 Zoology 、 Genomic library 、 Genetics 、 Endangered species 、 Locus (genetics) 、 Tidewater goby 、 Population bottleneck 、 Biology 、 Eucyclogobius
摘要: The first microsatellite loci for the endangered tidewater goby Eucyclogobius newberryi are described. A partial genomic library was constructed in pUC19 plasmid. Microsatellite-containing clones were isolated, sequenced, and amplified. Out of 12 positive 3 polymorphic proved useful a preliminary genetic screen well-separated populations (50 individuals each) along central California coast. number alleles per locus ranged from 2 to 3. This initial study suggests population bottleneck had occurred one populations.
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