Modulation of Second Messenger System on Ecliptae Herba Induced Ion Current in Single Neuronal Cell

摘要: The effects of Ecliptae Herba on ion channels single neuron employing nystatin perforated patch clamp under voltage were investigated and the following results obtained. 1. magnitudes currents caused by various concentrations compared to glycine used as control recorded. As result Herba, in neuron, recorded depending concentrations. With 10.0mg/ml current equivalent was it maximal concentration. 2. Considered 3mg/ml control, data 1) bicuculline suppressed . 2) tubocurarine repressed 3) When both applied, Therefore, from is due mainly excitation receptor rather than acetylcholine receptor. 3. Taken 1mg/ml D-AP5, a NMDA antagonist, CNQX, non-NMDA antagonist This experiment had shown that does not greatly affect glutamate receptors. 4. currents, when 0.5mg/ml applied two minutes interval, reduced proceeded. mixed solution forskolin, IBMX dibutyl cAMP for thirty seconds, more sharply control. Hence, this presented fact magnitude decreased concentration increased. 5. pre-treated staurosporin dully neurone, activated inhibited cAMP-protein kinase.