Reconstruction of the adenosine system by bone marrow-derived mesenchymal stem cell transplantation.
作者： Qi Hu , Suiqiang Zhu , Huicong Kang , Xiaoyan Liu , Feng Xu
DOI: 10.3969/J.ISSN.1673-5374.2012.04.002
关键词: Adenosine A1 receptor 、 Adenosine receptor 、 Bone marrow 、 Immunology 、 Transplantation 、 Medicine 、 Hippocampus 、 Adenosine A2A receptor 、 Pathology 、 Adenosine 、 Mesenchymal stem cell
摘要: In the present study, we transplanted bone marrow-derived mesenchymal stem cells into CA3 area of hippocampus chronic epilepsy rats kindled by lithium chloride-pilocarpine. Immunofluorescence and western blotting revealed an increase in adenosine A1 receptor expression a decrease A2a brain tissues epileptic 3 months after transplantation. Moreover, imbalance receptor/A2a ratio was improved. Electroencephalograms showed that frequency amplitude spikes frontal lobe were reduced. These results suggested cell transplantation can reconstruct normal function system greatly improve epileptiform discharges.
nrronline.org参考文章(26)
Annelies Van Dycke, Robrecht Raedt, Ine Dauwe, Tom Sante, Tine Wyckhuys, Alfred Meurs, Kristl Vonck, Wytse Wadman, Paul Boon, Continuous local intrahippocampal delivery of adenosine reduces seizure frequency in rats with spontaneous seizures Epilepsia. ,vol. 51, pp. 1721- 1728 ,(2010) , 10.1111/J.1528-1167.2010.02700.X
D. Boison, Adenosine as a modulator of brain activity. Drug News & Perspectives. ,vol. 20, pp. 607- 611 ,(2007) , 10.1358/DNP.2007.20.10.1181353
Detlev Boison, Louis Scheurer, Jack L. Tseng, Patrick Aebischer, Hanns Mohler, Seizure suppression in kindled rats by intraventricular grafting of an adenosine releasing synthetic polymer. Experimental Neurology. ,vol. 160, pp. 164- 174 ,(1999) , 10.1006/EXNR.1999.7209
P. Mareš, Anticonvulsant action of 2-chloroadenosine against pentetrazol-induced seizures in immature rats is due to activation of A1 adenosine receptors Journal of Neural Transmission. ,vol. 117, pp. 1269- 1277 ,(2010) , 10.1007/S00702-010-0465-9
Detlev Boison, Kerry-Ann Stewart, Therapeutic epilepsy research: From pharmacological rationale to focal adenosine augmentation Biochemical Pharmacology. ,vol. 78, pp. 1428- 1437 ,(2009) , 10.1016/J.BCP.2009.08.005
Nicholas Dale, Bruno Frenguelli, Release of Adenosine and ATP During Ischemia and Epilepsy Current Neuropharmacology. ,vol. 7, pp. 160- 179 ,(2009) , 10.2174/157015909789152146
Martin Güttinger, Vivianne Padrun, William F. Pralong, Detlev Boison, Seizure suppression and lack of adenosine A1 receptor desensitization after focal long-term delivery of adenosine by encapsulated myoblasts. Experimental Neurology. ,vol. 193, pp. 53- 64 ,(2005) , 10.1016/J.EXPNEUROL.2004.12.012
Detlev Boison, Alexander Huber, Vivianne Padrun, Nicole Déglon, Patrick Aebischer, Hanns Möhler, Seizure Suppression by Adenosine‐releasing Cells Is Independent of Seizure Frequency Epilepsia. ,vol. 43, pp. 788- 796 ,(2002) , 10.1046/J.1528-1157.2002.33001.X
Jana Dvorakova, Alena Hruba, Vladimir Velebny, Lukas Kubala, Isolation and characterization of mesenchymal stem cell population entrapped in bone marrow collection sets Cell Biology International. ,vol. 32, pp. 1116- 1125 ,(2008) , 10.1016/J.CELLBI.2008.04.024
Gaoying Ren, Tianfu Li, Jiang Quan Lan, Andrew Wilz, Roger P. Simon, Detlev Boison, Lentiviral RNAi-induced downregulation of adenosine kinase in human mesenchymal stem cell grafts: a novel perspective for seizure control. Experimental Neurology. ,vol. 208, pp. 26- 37 ,(2007) , 10.1016/J.EXPNEUROL.2007.07.016