Rapid amplification of complementary DNA ends for generation of full-length complementary DNAs: thermal RACE.
作者： Michael A. Frohman
DOI: 10.1016/0076-6879(93)18026-9
关键词: Genetics 、 RNA-Directed DNA Polymerase 、 cDNA library 、 Oligonucleotide 、 Genomic library 、 Complementary DNA 、 Transcription (biology) 、 Reverse transcriptase 、 Biology 、 Rapid amplification of cDNA ends
摘要: Publisher Summary The generation of a full-length complementary DNA (cDNA) can represent the most challenging part cloning project, particularly with respect to obtaining 5' end cDNA. initial step––reverse transcription messenger RNA (mRNA)—is also critical one, and many variations on it is described maximize likelihood fully extended Thermal rapid amplification cDNA ends (RACE) polymerase chain reaction (PCR) technique through which previously unobtained 3' be amplified starting knowledge small stretch sequence from within an internal region This provides alternative constructing screening conventional libraries obtain remainder for partially cloned Partial cDNAs are generated using PCR amplify copies between single point in mRNA transcript its or end. To use RACE protocol, short, must already known interest.
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