Response surface methodology: Synthesis of inulooligosaccharides with an endoinulinase from Aspergillus niger

摘要: Abstract Oligosaccharides of chain length up to degree polymerisation (DP) 5 have been synthesised using a purified endoinulinase and response surface methodology with central composite experimental design for the optimisation product yield. The was through three-step procedure in yield 1.11% 3.5-fold its molecular weight 68.1 kDa by SDS-PAGE. It had optimum pH temperature values 6 60 °C, respectively only 10% loss activity over 60 min under these conditions. From kinetic studies Km Vmax were estimated at 3.53 mM 666.7 μmol min−1 ml−1, respectively. Using preparation isolated from Aspergillus niger, (60 °C), inulin concentration (150 mg ml−1), incubation time (48 h), (6.0) enzyme dosage (60 U ml−1) produced high yields inulotrioses (70.3 mM), inulotetraoses (38.8 mM), inulopentaoses (3.5 mM). Response regression predicted similar levels same A comparative analysis individual oligosaccharides both crude is also presented.