LINC00319 promotes osteosarcoma progression by regulating the miR‐455‐3p/NFIB axis

摘要: Background Numerous studies have shown that aberrant expression of long non-coding RNAs (lncRNAs) is associated with the development and metastasis osteosarcoma (OS). However, role function LINC00319 respect to regulating OS progression unknown. The present study aimed reveal related mechanism in OS. Methods LINC00319, miR-455-3p nuclear factor IB (NFIB) cells tissues was determined using a reverse transcriptase-polymerase chain reaction (PCR). sublocalization predicted by lncATLAS database (http://lncatlas.crg.eu) RNA fluorescence situ hybridization (FISH) further performed detect subcellular localization LINC00319. NFIB target sites were StarBase (http://starbase.sysu.edu.cn/index.php) validated dual luciferase reporter gene assay. We subsequently gain- loss-of-function define cell migration. Results PCR results showed lncRNA exhibited high tumor tissue. Moreover, positioned cytoplasm, which identified FISH. Knockdown LINC00319/NFIB or overexpression blocked migration cells. In addition, inhibitory effect knockdown partially administration inhibitor. Conclusions may promote miR-455-3p/NFIB axis, probably serves as an innovative potential indicator prognosis therapy for