Convenient and versatile subcellular extraction procedure, that facilitates classical protein expression profiling and functional protein analysis.

摘要: Standardized sample preparation to reduce proteome complexity facilitates subsequent analysis. Here we describe a robust sequential extraction method that enables simple fractionation of proteins in their native state according subcellular localization, yielding four subproteomes enriched (a) cytosolic; (b) membrane and organelle-localized; (c) soluble DNA-associated nuclear (d) cytoskeletal proteins. Efficiency selectivity is demonstrated by morphological-, two-dimensional electrophoresis image-, immunological- as well enzymatic-analysis. In pilot studies, redistribution regulatory was successfully measured.