Molecular investigation of the genetic base of sugarcane cultivars

摘要: Molecular diversity was analysed among 162 clones of sugarcane using DNA restriction fragment length polymorphism (RFLP). One hundred and nine them were modern cultivars interspecific origin; most bred in Barbados or Mauritius. Fifty three from Saccharum officinarum species, which is the major source genes cultivars, prevailing over part genome incorporated wild species spontaneum. Twelve low-copy nuclear probes scattered used combination with one two enzymes. A total 399 fragments identified, 386 polymorphic. Each clone displayed a high number per probe/enzyme combination, illustrating polyploid constitution genome. Among S. clones, those New Guinea had largest variability encompassed that present collected Indonesian Islands known to have been involved parentage cultivars. This agreement hypothesis centre origin this species. The Caledonia formed separate group could correspond modified through introgression other members ‘Saccharum complex’. Despite low for breeding more than 80% markers whole sample also found due probably heterozygosity related polyploidy. main groups, originating Mauritius, clearly separated. appeared essentially spontaneum alleles Mauritian absent Barbadan ones, relation regular use early generation hybrids program employed