A high genetic diversity revealed between commercial rose cultivars by RAPD-PCR technique

作者: M. Emadpour , E. Beiramizadeh , R. Hosseini

DOI: 10.22069/IJPP.2012.664

关键词: UPGMACultivarJaccard indexGenetic diversityVeterinary medicineRAPDDendrogramSimilarity (network science)BotanyBiologyGenetic marker

摘要: Genetic diversity between fifteen commercial cultivars of rose were evaluated, using Ten decamer primers (A-J). All detected polymorphism among the cultivars. In total, 126 bands produced, 73 which polymorphic. Primers E and F produced highest, while primer H lowest number bands. The percentage polymorphic ranged from 37% to 81% with an average 63.9%. was 7.3 per primer. Only amplified DNA fragments ranging in size 220 3000 bp used for statistical analyses. Cluster analysis based on presence or absence performed by Jaccard’s similarity coefficient, Unweighted Pair Group Method Arithmetic Averages (UPGMA). 0.12 0.53. dendrogram revealed two main clusters. Each cluster divided into subgroups. This investigation showed that genetic relatively considerable these Also, results propose RAPD marker is a useful tool evaluation relationships amongst different

参考文章(16)
N. Vijayan Nair, Suresh Nair, T.V. Sreenivasan, Madan Mohan, Analysis of genetic diversity and phylogeny in Saccharum and related genera using RAPD markers Genetic Resources and Crop Evolution. ,vol. 46, pp. 73- 79 ,(1999) , 10.1023/A:1008696808645
A. H. Jürgens, B. Seitz, I. Kowarik, Genetic differentiation of Rosa canina (L.) at regional and continental scales Plant Systematics and Evolution. ,vol. 269, pp. 39- 53 ,(2007) , 10.1007/S00606-007-0569-3
Qiang Xu, Xiaopeng Wen, Xiuxin Deng, A simple protocol for isolating genomic DNA from chestnut rose (Rosa roxburghii tratt) for RFLP and PCR analyses Plant Molecular Biology Reporter. ,vol. 22, pp. 301- 302 ,(2004) , 10.1007/BF02773140
Anuradha Mohapatra, Gyana Ranjan Rout, Identification and Analysis of Genetic Variation among Rose Cultivars Using Random Amplified Polymorphic DNA Zeitschrift für Naturforschung C. ,vol. 60, pp. 611- 617 ,(2005) , 10.1515/ZNC-2005-7-817
Alireza Babaei, Seyed Reza Tabaei-Aghdaei, Morteza Khosh-Khui, Reza Omidbaigi, Mohammad Reza Naghavi, Gerhard D Esselink, Marinus JM Smulders, Microsatellite analysis of Damask rose ( Rosa damascena Mill.) accessions from various regions in Iran reveals multiple genotypes BMC Plant Biology. ,vol. 7, pp. 12- 12 ,(2007) , 10.1186/1471-2229-7-12
Nilgün Göktürk Baydar, Hasan Baydar, Thomas Debener, Analysis of genetic relationships among Rosa damascena plants grown in Turkey by using AFLP and microsatellite markers. Journal of Biotechnology. ,vol. 111, pp. 263- 267 ,(2004) , 10.1016/J.JBIOTEC.2004.04.014
S. Matsumoto, H. Fukui, Identification of rose cultivars and clonal plants by random amplified polymorphic DNA Scientia Horticulturae. ,vol. 67, pp. 49- 54 ,(1996) , 10.1016/S0304-4238(96)00951-X
J. M. McGrath, C. A. Derrico, Y. Yu, Genetic diversity in selected, historical US sugarbeet germplasm and Beta vulgaris ssp. maritima. Theoretical and Applied Genetics. ,vol. 98, pp. 968- 976 ,(1999) , 10.1007/S001220051157
P.K. Bretting, M.P. Widrlechner, Genetic markers and horticultural germplasm management Hortscience. ,vol. 30, pp. 1349- 1356 ,(1995) , 10.21273/HORTSCI.30.7.1349