Up-regulating effect of hepatitis C virus core protein on expression of cell cycle gene Wee1
作者： Jian-Jun Wang , Yan Liu , Jun Cheng , Qian Yang , Yan-Jie Yang
关键词: Gene 、 genomic DNA 、 Virology 、 Cell culture 、 FuGENE 、 Transfection 、 Polymerase chain reaction 、 Molecular biology 、 Promoter 、 Biology 、 XhoI
摘要: AIM: To investigate the transactivating effect of HCV core protein on Wee1 gene and molecular biological mechanisms in pathogenicity. METHODS: Polymerase chain reaction (PCR) technique was employed to amplify sequence promoter from HepG2 genomic DNA, product cloned into pGEM-T vector. The Weep cut T-Weep by KpnI XhoI, then pCAT3 basic, named pCAT3- Weep. pCAT3-Weep transfected hepatoblastoma cell line cotransfected cells with pcDNA3.1(-)-core FuGENE 6 transfection reagents. pCAT3-basic used as negative control. activity CAT detected an ELISA kit after 48 hours, which reflected function promoter.
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