Strain-specific differences in formation of apoptotic DNA ladders in MCF-7 breast cancer cells
作者： Jennifer L Gooch , Douglas Yee
DOI: 10.1016/S0304-3835(99)00208-6
关键词: Doxorubicin 、 Fragmentation (cell biology) 、 Apoptosis 、 DNA laddering 、 Biology 、 DNA fragmentation 、 Programmed cell death 、 Molecular biology 、 Immunology 、 DNA 、 Apoptotic DNA fragmentation 、 Cancer research 、 Oncology
摘要: We tested the ability of seven MCF-7 strains to undergo DNA fragmentation, as measured by laddering, following doxorubicin-induced apoptosis. Four were found laddering while three not. All inhibited doxorubicin, although sensitivity differed. Finally, we show detection sub-G1 that doxorubicin induced same fold increase in apoptosis MCF-7-ATCC, which did not ladder, and MCF-7-MG, ladder. Therefore, ladders is an accurate indicator cells fragmentation appears vary between strains.
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